Projectscarotenoid synthesis

CartAccnoStrainnameFeatures
P30795YEplac195-YB/Iyeast episomal plasmid derived from YEplac195, transformation marker URA3, expressing bifunctional crtYB (phytoene synthase and Lycopene cyclase) and crtI (phytoene desaturase) from Xanthophyllus dendrorhous
P30796YEplac195-YB/I/BTS1yeast episomal plasmid derived from YEplac195, transformation marker URA3, expressing bifunctional crtYB (phytoene synthase and Lycopene cyclase) and crtI (phytoene desaturase) from Xanthophyllus dendrorhous and Saccharomyces cerevisiae BTS1 (geranylgeranyl diphosphate synthase, homologous overexpression)
P30797YEplac195-YB/I/Eyeast episomal plasmid derived from YEplac195, transformation marker URA3, expressing bifunctional crtYB (phytoene synthase and Lycopene cyclase), crtI (phytoene desaturase), and ctrE (geranylgeranyl diphosphate synthase) from Xanthophyllus dendrorhous
P30798YIplac211-YB/I/BTS1yeast integrazive plasmid derived from YIplac211,ransformation marker URA3, expressing bifunctional crtYB (phytoene synthase and Lycopene cyclase) and crtI (phytoene desaturase) from Xanthophyllus dendrorhous and Saccharomyces cerevisiae BTS1 (geranylgeranyl diphosphate synthase, homologous overexpression)
P30799YIplac211-YB/I/Eyeast integrative plasmid derived from YIplac211, transformation marker URA3, expressing bifunctional crtYB (phytoene synthase and Lycopene cyclase), crtI (phytoene desaturase), and ctrE (geranylgeranyl diphosphate synthase, A551G mutation) from Xanthophyllus dendrorhous
P30800YIplac128-crtIyeast integrative plasmid derived from YIplac128, transformation marker LEU2, expressing crtI (phytoene desaturase) from Xanthophyllus dendrorhous
P30801YIplac204-tHMG1yeast integrative plasmid derived from YIplac204, transformation marker TRP1, expressing truncated Saccharomyces cerevisiae HMG1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase, C-terminal 524 aa)
Y41387RVFG039yeast strain derived from CEN.PK: CEN.PK, MATa, ura3-52::URA3-crtYB-crtI-crtE*[YIplac211-YB/I/E*], SUC2, MAL2-8c
Y41388RVFG123yeast strain derived from CEN.PK: CEN.PK, MATa, ura3-52::URA3-crtYB-crtI-crtE*[YIplac211-YB/I/E*], leu2-3,112, trp1-289, SUC2, MAL2-8c
Y41389RVFG128yeast strain derived from CEN.PK: CEN.PK, MATa, ura3-52::URA3-crtYB-crtI-crtE*[YIplac211-YB/I/E*], leu2-3,112::LEU2-crtI[YIplac128-crtI], trp1-289, SUC2, MAL2-8c

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carotenoid synthesis at SGD