ProjectsEllenberg plasmids

CartAccnoStrainnameFeatures
P30453pLBR1TM-GFPThis LBR–GFP fusion includes the first 238 amino acids of human lamin B receptor fused to the F64L, S65T, H231L variant of the GFP with a four–amino acid spacer (PVAT).
P30454pLBR1TM-YFPThis LBR–GFP fusion includes the first 238 amino acids of human lamin B receptor fused to the S65G, V68L, S72A, T203Y, H231L variant of the GFP (YFP) with a four–amino acid spacer (PVAH).
P30455pEGFP-LaminB1N-terminal EGFP fusion of human laminB1
P30456pCFP-LaminB1N-terminal ECFP fusion of human laminB1
P30457pEGFP-Nup153N-terminal EGFP fusion of human Nup153. Only one copy of EGFP fused to Nup153!
P30458pEGFP3-Nup153N-terminal fusion of 3 copies of EGFP to human Nup153
P30459pPOM121-EGFP3C-terminal fusion of 3 copies of EGFP to POM121
P30460pSRb-ECFPC-terminal fusion of SR beta to ECFP
P30461pgammaTubulin-DsRed1gamma tubulin C-terminal fused to DsRed1
P30462pYFP-LaminB1YFP N-terminal fused to LaminB1
P30463pEGFP-LAP2bEGFP N-terminal fused to lamina associated polypeptide 2 beta
P30464pYFP-LAP2bYFP N-terminal fused to lamina associated polypeptide 2 beta
P30465pYFP-alphaTubulinhYFP N-terminal fused to alpha-tubulin
P30466pH2B-DiHcRed1human histone H2B C-terminal fused to the dimer of the far red flourscent protein HcRed1
P30467pH2B-YFPhuman histone H2B C-terminal fused to YFP
P30468pEGFP-CENPAEGFP N-terminal fused to centromere specific variant of histone H3, C-terminal HA tagged
P30469pGEMHE-EGFP-LaminB1plasmid for invitro transcription of EGFP N-terminal fused to laminB1 after linearization with NheI
P30470pGEMHE-EGFP-Nup98plasmid for invitro transcription of EGFP N-terminal fused to human Nup98 after linearization with NheI or SphI
P30471pGEMHE-EGFP-Nup153plasmid for invitro transcription of EGFP N-terminal fused to human Nup153 after linearization with NheI or SphI
P30472pNup214-EGFPhuman Nup214 C-terminal fused to EGFP
P30473pGEMHE-EGFP-Nup214plasmid for invitro transcription of EGFP N-terminal fused to Nup214 after linearization with SphI
P30474pGEMHE-POM121-EGFP3plasmid for invitro transcription of POM121 fused to 3 copies of EGFP after linearization with NheI or SphI
P30475pEGFP-hALADINN-terminal EGFP fusion of hAladin
P30476pCG1-EGFP3C-terminal fusion of 3 EGFP copies to human CG1
P30477pGp210-EGFP3rGP210 insertion of 3 copies of EGFP between transmembrane und lumeral domains
P30478pEGFP-ImportinB1EGFP N-terminal fused to human importin B1
P30479pEGFP-Nup35human Nup35 N-terminal EGFP fusion
P30480pEGFP-Nup37human Nup37 N-terminal EGFP fusion
P30481pEGFP-Nup43human Nup43 N-terminal EGFP fusion
P30482pEGFP2-Nup50mNup50 N-terminal fusion to 2 EGFP copies
P30483pEGFP2-Nup58human Nup58 N-terminal fusion to 2 EGFP copies
P30484pNup62-EGFP3mNup62 C-terminal fusion to 3 copies of EGFP
P30485pEGFP3-Nup85human Nup85 N-terminal fusion to 3 EGFP copies
P30486pNup93-EGFP3human Nup93 C-terminal fusion to 3 EGFP copies
P30487pEGFP-Nup98human Nup98 N-terminal EGFP fusion
P30488pEGFP-Nup214human Nup214 N-terminal fusion to EGFP
P30489pSec13-EGFP3human Sec13 C-terminal fusion with 3 EGFP copies
P30490pEGFP2-Seh1human Seh1 N-terminal fusion to 2 EGFP copies
P30491pGEMHE-EGFP-ABDplasmid for invitro transcription of the actin binding domain of Dictyostelium ABP120 N-terminal fused to EGFP, linearization with NheI or SphI
P30492pGEMHE-ensconsin-EGFP3plasmid for invitro transcription of ensconsin C-terminal fused to 3 EGFP copies. No DNA sequence provided by the constructing lab!
P30493pGEMHE-H2B-DiHcRed1plasmid for invitro transcription of the histone H2B C-terminal fused to the intramolecular dimer of the far-red flourescent protein HcRed1, linearization with NheI or SphI
P30494pbeta-gal-DiHcRed1E.coli beta-galactosidase C-terminal fusion to the intramolecular dimer of far-red fluorescent protein HcRed1
P30495pH1.2-PAGFPisoform 2 of histone H1 C-terminal fusion to photoactivatable green fluorescent protein
P30496pH1.3-PAGFPisoform 3 of histone H1 C-terminal fusion to photoactivatable green fluorescent protein
P30497pH1.4-PAGFPisoform 4 of histone H1 C-terminal fusion to photoactivatable green fluorescent protein
P30498pH1.5-PAGFPisoform 5 of histone H1 C-terminal fusion to photoactivatable green fluorescent protein
P30499pH2B-PAGFPhistone H2B C-terminal fusion to photoactivatable green fluorescent protein
P30500pEGFP-Hp1-bhuman heterchromatin protein1 beta N-terminal fusion to EGFP
P30501pEGFP-RCC1guanine nucleotide exchange factor RCC1 N-terminal fusion to EGFP
P30502pPAGFP-RCC1guanine nucleotide exchange factor RCC1 N-terminal fusion to photoactivatable green fluorescent protein
P30503pH1.1-PAGFPisoform 1 of histone H1 C-terminal fusion to photoactivatable green fluorescent protein
P30504pEGFP-Scc1human Scc1 subunit of cohesin complex fusion with N-terminal FLAGtag and EGFP
P30505pEGFP-SA1human SA1 subunit of cohesin complex fusion to N-terminal FLAG tag and EGFP
P30506pEGFP-Kleisin-gammaKleisin-gamma subunit of the condensin I complex with N-terminal FLAG-tag and EGFP fusion
P30507pEGFP-Kleisin-betaKleisin-beta subunit of the condensin I complex with N-terminal FLAG-tag and EGFP fusion
P30508pH2B-mRFPhuman histone H2b C-terminal fusion to monomeric red fluorescent protein
P30509pMAGT-H2Bhuman histone H2B N-terminal fusion to mutated variant of O6-alkylguanine-DNA alkyltransferase
P30510p4LambdaN22-3mEGFP-M94 lambda N22-3 monomeric EGFP-M9 nuclear localization sequence-HIStag; lamdba N22 peptide binds to the B-box of tagged mRNA
P30511pmRFP-4boxB-beta-actin-zipcodemonomeric red fluorscent protein mRNA fused to 4 boxB and beta action zipcode in the 3'UTR, boxB in the 3'UTR enables binding of lambda peptide N22 to the mRNA, beta actin zipcode in the 3'UTR directs the mRNA into the cytosol
P30512pSRb-mRFP-4boxBsignal recognition particle beta subunit fused monomeric red fluorscent protein with mRNA containing 4 boxB in the 3'UTR, boxB in the 3'UTR enables binding of lambda peptide N22 to the mRNA, signal recognition particle beta subunit directs the fused mRFP into the ER membrane
P30513p5boxB-SRPRNAhuman SRP mRNA under homologues promoter and terminator, in the 5'UTR fused to 5 copies of boxB enabling binding of lambda N22 to the mRNA
P30514pSRP19-mRFPsignal recognition particle component SRP19 C-terminal fusion to monomeric red fluorescent protein
P30515pEGFP-H2Bhuman histone H2B N-terminal fusion to EGFP
P30516pmEGFP-alpha-tubulinalpha tubulin N-terminal fusion to monomeric EGFP
P30517pGEMHE-H2B-mRFPplasmid for invitro transcription of the histone H2B C-terminal fused to monomeric red flourescent protein, linearization with AscI
P30518pGEMHE-EGFP-MAP4plasmid for invitro transcription of the microtuble-associated protein 4 N-terminal fused to EGFP, linearization with SphI or NheI
P30519pGEMHE-EB-mEGFPplasmid for invitro transcription of human end binding protein 3 C-terminal fused to monomeric EGFP, linearization with AscI
P30520pGEMHE-C-moesin-mRFPplasmid for invitro transcription of f-actin binding domain of moesin C-terminal fused to monomeric red fluorescent protein 1, linearization with SphI or NheI
P30551pSRb-mRFP-16boxBsignal recognition particle beta subunit fused monomeric red fluorscent protein with mRNA containing 16 boxB copies in the 3'UTR, boxB in the 3'UTR enables binding of lambda peptide N22 to the mRNA, signal recognition particle beta subunit directs the fused mRFP into the ER membrane
P30552pmRFP-16boxB-beta-actin-zipcodemonomeric red fluorscent protein mRNA fused to 16 boxB copies and beta action zipcode in the 3'UTR, boxB in the 3'UTR enables binding of lambda peptide N22 to the mRNA, beta actin zipcode in the 3'UTR directs the mRNA into the cytosol
P30553pIBB-DiHcRed1importin beta – binding domain of importin alpha C-terminal fused to the dimer of the far red flourscent protein HcRed1
P30554pPOM121-mCherryhuman POM121 C-terminal fused to mCherry
P30606pLBR1TM-mCherryThis LBR–mCherry fusion includes the first 238 amino acids of human lamin B receptor fused to mCherry
P30607pCS2-Fmn2-mCherryplasmid for invitro transcription of Formin-2 C-terminal fused to mCherry, linearization with BssHII (or NsiI)
P30608pGamma-tubulin-SNAP-taggamma-tubulin C-terminal fused to SNAP-tag
P30609pEB3-mCherryhuman end binding protein 3 C-terminal fused to mCherry
P30610pmCherry-MAP4mCherry N-terminal fused to microtuble-associated protein 4
P30613pmEGFP-PB2monomeric enhanced green fluorescent protein N-terminal fused to PB2 subunit of the trimeric influenza virus RNA polymerase
P30614pmEGFP-PB2_K738Q_K752N_R755Qmonomeric enhanced green fluorescent protein N-terminal fused to PB2 subunit of the trimeric influenza virus RNA polymerase modified at K738Q K752N R755Q
P30615pPB1-mEGFPPB1 subunit of the trimeric influenza virus RNA polymerase C-terminal fused to monomeric enhanced green fluorescent protein
P30616pPA-mCherryPA subunit of the trimeric influenza virus RNA polymerase C-terminal fused to mCherry
P30617pPB1-mCherryPB1 subunit of the trimeric influenza virus RNA polymerase C-terminal fused to mCherry
P30618pmCherry-PB2mCherry N-terminal fused to PB2 subunit of the trimeric influenza virus RNA polymerase
P30619pPA-mCFPPA subunit of the trimeric influenza virus RNA polymerase C-terminal fused to monomeric cyan fluorescent protein
P30620pPB1-mCFPPB1 subunit of the trimeric influenza virus RNA polymerase C-terminal fused to monomeric cyan fluorescent protein
P30621pmCFP-PB2monomeric cyan fluorescent protein N-terminal fused to PB2 subunit of the trimeric influenza virus RNA polymerase
P30622pmEGFP-MBP-mCherry monomeric enhanced green fluorescent protein N-terminal fused to maltose binding protein C-terminal fused to mCherry
P30623pmEGFP2tandem monomeric enhanced green fluorescent protein
P30624pmEGFP5fusion of 5 copies of monomeric enhanced green fluorescent protein
P30625pmEGFP10fusion of 10 copies of monomeric enhanced green fluorescent protein
P30626pmRFP2tandem monomeric red fluorescent protein
P30627pmPAGFP2tandem monomeric photoactivatable green fluorescent protein
P30628pmPAGFP-H1tmonomeric photoactivatable green fluorescent protein N-terminal fused to last 70 aa of of isoform 1 of the linker histone H1
P30629pMBP-EGFPmaltose binding protein C-terminal fused to enhanced green fluorescent protein
P30630pEGFP-M9enhanced green fluorescent protein fused to M9-His tag (M9 is a relatively weak NLS)
P30631pIBB-EGFPimportin-beta-binding domain of importin alpha C-terminal fused to enhanced green fluorescent protein (IBB is a strong NLS)
P30632pH2B-mCherryHistone H2B C-terminal fused to mCherry mutant fluorescent protein derived from the tetrameric Discosoma sp. red flurescent protein DsRed; coding sequence has been human codon-optimized"
P30648pPOM121-3mCherryrattus norvegicus POM121 C-terminal fused to 3 copies of mCherry mutant fluorescent protein derived from the tetrameric Discosoma sp. red flurescent protein DsRed
P30649p3mCherry-Nup1073 copies of mCherry mutant fluorescent protein derived from the tetrameric Discosoma sp. red flurescent protein DsRed (coding sequence has been human codon-optimized) N-terminal fused to human Nup107
P30650pNUP153-EGFPC-terminal fusion of human NUP153 to EGFP
P30651pMS2-H2B-EGFPHistone H2B C-terminal fused to enhanced green fluorescent protein. No DNA sequence information available
P30652pGEMHE-H2B-mCherryHistone H2B C-terminal fused to mCherry
P30653pGEMHE-3mEGFP-UtrCH
P30659pGEMHE-EGFP-CENP-C1enhanced green fluorescent protein N-terminal fused to mus musculus CENP-C1 , linearization with AscI or BssHII
P30660pGEMHE-3mCherry-CENP-C13 copies of mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein DsRed (coding sequence has been human codon-optimized) N-terminal fused to mus musculus CENP-C1 , linearization with AscI or BssHII
P30661pGEMHE-Mad2-mEGFPmonomeric enhanced green fluorescent protein C-terminal fused to homo sapiens MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1), linearization with AscI or BssHII
P30728pmEGFP-Nup133-s31401resmonomeric enhanced green fluorescent protein N-terminal fused to Nup133, siRNA resistant (s31401res)
P30729pEGFP-Nup107-s32727resenhanced green fluorescent protein N-terminal fused to Nup107, siRNA resistant (s32727res)
P30730pmEGFP-Nup85-s36612resmonomeric enhanced green fluorescent protein N-terminal fused to Nup85, siRNA resistant (s36612res)
P30731pmEGFP-Seh1-s37879resmonomeric enhanced green fluorescent protein N-terminal fused to Seh1, siRNA resistant (s37879res)
P30732pmEGFP-Nup160-s23466resmonomeric enhanced green fluorescent protein N-terminal fused to Nup160, siRNA resistant (s23466res)
P30733pNup160-s23466res-mEGFPNup160, siRNA resistant (s23466res), C-terminal fused to monomeric enhanced green fluorescent protein
P30734pEGFP-Nup37-s35439resenhanced green fluorescent protein N-terminal fused to Nup37, siRNA resistant (s35439res)

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Ellenberg plasmids at SGD