pGEMHE-POM121-EGFP3(P30474) Ellenberg plasmids
Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes. Lénárt P, Rabut G, Daigle N, Hand AR, Terasaki M, Ellenberg J. J Cell Biol (2003) 160, 1055-1068
Transformed E. coli cells are resistant to ampicillin.
plasmid for invitro transcription of POM121 fused to 3 copies of EGFP after linearization with NheI or SphI